Feb 29, 2024  
2013-2014 Course Catalog 
2013-2014 Course Catalog [ARCHIVED CATALOG]

Biotechnology Laboratory Methods

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The Biotechnology Laboratory Methods certificate is designed for students who currently have previous coursework or experience equivalent to the core biotechnology AA  or AS  program courses in biology and chemistry to quickly obtain additional laboratory training and update their current skills in order to broaden their job position or to obtain employment in biotechnology. The certificate includes the Biotechnology program capstone courses BIO 250 - Cell & Molec Bio-Nucleic Acids , BIO 251 - Cell & Molecular Bio-Proteins  and Microbiology to provide them with more than 200 hours of hands-on lab experience, as well as Statistics to ensure graduates are able to interpret scientific data.

The certificate coursework is structured to allow students to develop marketable job skills, focusing on written and oral communications, critical thinking, problem-solving, computer skills and small-group collaboration. The hands-on laboratory work enables students to develop understanding and proficiency in the following wide variety of biotechnology laboratory methods:

Nucleic Acids Protein Chemistry Microbiology

Laboratory Safety Computer Modeling of Protein Structures and Interactions Oil Immersion Microscopy
Laboratory Notebooks and Documentation Solution Preparation Aseptic Transfers and Inoculation
Solution Preparation Plant Tissue Culture Measure Total Protein in Food Samples Wet Mount and Hanging Drop Preparations
Restriction Enzymes Measure Enzyme Activity Staining Techniques: Simple, Negative, Gram, Acid-Fast, Capsule, Endospore, Flagella
Restriction Digestion Analysis Test Effects of Concentrations, pH and Temperature on Enzymes
Classic PCR Chromatography Methods: Colony Morphology Analysis
Real-time PCR   Size Exclusion Isolation Methods Selective Media Analysis Metabolic Analysis:
Agarose Gel Electrophoresis   Affinity   Carbohydrate, Protein
STR Analysis   Ion Exchange Identification of Unknown Microorganism
gDNA Extraction Polyacrylamide Gel Electrophoresis Antimicrobial Susceptibility Analysis
GMO Food Testing Protein Extraction & Purification Plaque Assay and Analysis
Gene Cloning Analysis of Extracted Protein Total Coliform Determination
Nested PCR   SDS-PAGE  
PCR Product Purification Enzyme Activity  
Ligation into Plasmid vectors ELISA and Automation of ELISA’s  
Clone Sequence Analysis Western Blotting  
Bioinformatics 2-D PAGE  
Southern Blotting    

Please check with the program chairperson for Biotechnology or an advisor for additional information or assistance. For more information about the Biotechnology program, please visit our website at www.dmacc.edu/programs/biotechnology.

Locations: Ankeny

Selected courses in this program are offered at other campuses.

Program Entry Requirements

  1. Complete an application for admission.
  2. Satisfy the assessment requirement.
  3. Attend any required information/registration session.
  4. BIO 112  and BIO 113 .
  5. CHM 122  and CHM 132  OR CHM 165  and CHM 175 .

Students start Fall or Spring semester.

Graduation Requirements

To earn a Biotechnology Laboratory Methods certificate, a student must be accepted into the Biotechnology Laboratory Methods certificate program, complete all course work as prescribed and maintain a 2.0 grade point average.

Required Courses

Total Credits Required to Complete this Certificate - 18

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