BIO 186 - Microbiology

1. Perform microbiological laboratory procedures
   1. Understand and comply with safety and health rules of the microbiology laboratory
   2. Perform all microbiological laboratory procedures according to appropriate safety standards.
   3. Demonstrate an understanding of the use and care of laboratory microscopes including use of oil immersion.
   4. Utilize proper techniques in the isolation and culturing of bacteria.
   5. Employ and understanding the importance of aseptic technique.
   6. Perform microbiological staining procedures including the Gram, acid-fast, negative, endospore, flagella, and capsule stains.
   7. Differentiate between Gram-negative and Gram-positive bacteria
   8. Utilize selective, differential, and enrichment media for isolating bacteria.
   9. Isolate bacteria from the environment.
   10. Understand and employ the use of biochemical tests in the identification of microbes.
   11. Use appropriate stains, chemical media, and techniques to identify unknown microbes.
   12. Perform antibiotic sensitivity assessment of bacteria isolates.
   13. Demonstrate the use and effectiveness of antiseptics and disinfectants.
   14. Use serial dilutions to quantify microorganisms.
   15. Demonstrate and quantify the presence of microorganisms in food and water samples.
   16. Understand and demonstrate the use of microorganisms in biotechnology and molecular biology
2. Understand the history and scope of the field of microbiology.
3. Explain the types, structures and functions of major classes of biological macromolecules.
4. Demonstrate knowledge of prokaryotic and eukaryotic cell structures and their functions.
5. Identify the modes of microbial nutrition.
6. Describe the major cellular metabolic reactions of microbial cells.
   1. Glycolysis
   2. Fermentation
   3. Respiration
   4. Photosynthesis
7. Discuss microbial growth.
   1. Describe influence of environmental factors including temperature, pH, salinity, and oxygen concentration on growth.
   2. Describe batch and continuous culturing of microorganisms.
8. Explain the processes involved in the flow of genetic information within the microbial cell.
   1. DNA replication
   2. Transcription
   3. Translation
   4. Regulation of gene expression
9. Describe methods of microbial genetic recombination
   1. Transformation
   2. Tranduction
   3. Conjugation
10. Demonstrate an understanding of the use of microbes in biotechnology and genetic engineering.
11. Explain key viral concepts
    1. Viral classification
    2. Viral structure
    3. Life cycle of viruses
    4. Pathogenicity of viruses
12. Understand and discuss the diversity of bacterial organisms.
13. Describe concepts relating to infectious disease
    1. Host defenses
    2. Virulence
    3. Pathogenesis of disease
    4. Epidemiology
    5. Normal flora
    6. Identification of disease-producing organisms
    7. Treatment of infectious diseases
14. Describe the methods for the control of microbial growth.
    1. Physical methods
    2. Chemical methods
15. Identify the major classes of antimicrobial agents
    1. Mechanism of antimicrobial action
    2. Development and mechanism of antibiotic resistance
16. Demonstrate knowledge of basic immunology
17. Identify the key characteristics of the major groups of eukaryotic microorganisms
    1. Fungi
    2. Protozoa
    3. Algae
18. Discuss and describe the microorganisms associated with disease in the 11 body systems.
    1. Identify specific organisms with diseases seen in current health watches.
19. Explain major concepts in environmental and industrial microbiology
    1. Cycling of elements and nutrients in the environment
    2. Use of microorganisms in industrial processes.