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Nov 26, 2024
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BIO 186 - Microbiology Credits: 4 Lecture Hours: 3 Lab Hours: 3 Practicum Hours: 0 Work Experience: 0 Course Type: Core A general microbiology course with laboratory designed for the science major. Emphasis is placed on morphology, physiology, microbial genetics, virology and basic immunology and applications. Prerequisite: one semester of any college-level biology. Competencies
- Perform microbiological laboratory procedures
- Understand and comply with safety and health rules of the microbiology laboratory
- Perform all microbiological laboratory procedures according to appropriate safety standards.
- Demonstrate an understanding of the use and care of laboratory microscopes including use of oil immersion.
- Utilize proper techniques in the isolation and culturing of bacteria.
- Employ and understanding the importance of aseptic technique.
- Perform microbiological staining procedures including the Gram, acid-fast, negative, endospore, flagella, and capsule stains.
- Differentiate between Gram-negative and Gram-positive bacteria
- Utilize selective, differential, and enrichment media for isolating bacteria.
- Isolate bacteria from the environment.
- Understand and employ the use of biochemical tests in the identification of microbes.
- Use appropriate stains, chemical media, and techniques to identify unknown microbes.
- Perform antibiotic sensitivity assessment of bacteria isolates.
- Demonstrate the use and effectiveness of antiseptics and disinfectants.
- Use serial dilutions to quantify microorganisms.
- Demonstrate and quantify the presence of microorganisms in food and water samples.
- Understand and demonstrate the use of microorganisms in biotechnology and molecular biology
- Understand the history and scope of the field of microbiology.
- Explain the types, structures and functions of major classes of biological macromolecules.
- Demonstrate knowledge of prokaryotic and eukaryotic cell structures and their functions.
- Identify the modes of microbial nutrition.
- Describe the major cellular metabolic reactions of microbial cells.
- Glycolysis
- Fermentation
- Respiration
- Photosynthesis
- Discuss microbial growth.
- Describe influence of environmental factors including temperature, pH, salinity, and oxygen concentration on growth.
- Describe batch and continuous culturing of microorganisms.
- Explain the processes involved in the flow of genetic information within the microbial cell.
- DNA replication
- Transcription
- Translation
- Regulation of gene expression
- Describe methods of microbial genetic recombination
- Transformation
- Tranduction
- Conjugation
- Demonstrate an understanding of the use of microbes in biotechnology and genetic engineering.
- Explain key viral concepts
- Viral classification
- Viral structure
- Life cycle of viruses
- Pathogenicity of viruses
- Understand and discuss the diversity of bacterial organisms.
- Describe concepts relating to infectious disease
- Host defenses
- Virulence
- Pathogenesis of disease
- Epidemiology
- Normal flora
- Identification of disease-producing organisms
- Treatment of infectious diseases
- Describe the methods for the control of microbial growth.
- Physical methods
- Chemical methods
- Identify the major classes of antimicrobial agents
- Mechanism of antimicrobial action
- Development and mechanism of antibiotic resistance
- Demonstrate knowledge of basic immunology
- Identify the key characteristics of the major groups of eukaryotic microorganisms
- Fungi
- Protozoa
- Algae
- Discuss and describe the microorganisms associated with disease in the 11 body systems.
- Identify specific organisms with diseases seen in current health watches.
- Explain major concepts in environmental and industrial microbiology
- Cycling of elements and nutrients in the environment
- Use of microorganisms in industrial processes.
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